THAI NGUYEN UNIVERSITY UNIVERSITY OF AGRICULTURE AND FORESTRY NGUYEN THI THU THAO RESEARCH OF THE INSTANT TEA PRODUCTION PROCESS FORM MYXOPYRUM SMILACIFOLIUM BLUME PLANT BACHELOR THESIS Study Mode : Full-time Major : Food Technology Faculty : Advanced education program office Batch : 2016 - 2020 Thai Nguyen, 2020 i Luan van Thai Nguyen University of Agriculture and Forestry Degree Program Bachelor of Food Technology Student name NGUYEN THI THU THAO Student ID DTN1654190015 Research of the instant tea production process from Thesis Title Myxopyrum smilacifolium Blume plant Supervisor MsC. Dinh Thi Kim Hoa Supervisor’s signature Abstract: The research focused on the process of producing instant tea from Myxopyrum smilacifolium plants with a laboratory scale, the research extraction method for extracting total polysaccarides from Myxopyrum smilacifolium root. The instant tea product was made from solid Myxopyrum smilacifolium root extract and other herbal extracts with the sensory quality was evaluated by the sensory panel and had the quite good quality. Keywords : Myxopyrum smilacifolium, instant tea, process, product Number of pages: Date of submission: ii Luan van ACKNOWLEAGEMENT I would like to express my sincere thanks to the teachers of Thai Nguyen University of Agriculture and Forestry and especially the and teachers in the AEP Faculty, the Faculty of Biotechnology and Food Technology for giving me the opportunity to interact and learn asked during the four years.
I would like to thank Ms. Dinh Thi Kim Hoa, a lecturer in the Department of Biotechnology and Food Technology, who always gave me a wholehearted guidance in the process of studying and doing graduate research. I would like to thank teacher Luu Hong Son and teachers and friends in the Department of Food Technology Biotechnology for guiding and supplementing my knowledge of laboratory skills, always creating favorable conditions for you in the research process. Finally, I am extremely grateful to my family and friends for cheering me on during my graduation thesis.
Thank you sincerely! Thai Nguyen, December, 02, 2020 Sincerely Nguyen Thi Thu Thao iii Luan van TABLE OF CONTENTS Acknowleagement. iii Table of contents. iv List of table. vii List of figuges.
ix List of abbreviations. Scientific and practical significance. The scientific meaning of the topic. The practical meaning of the topic.
Overview about Myxopyrum smilacifolium (Wall. Characteristics of Myxopyrum smilacifolium (Wall.2 Overview of the chemical compositions of Myxopyrum smilacifolium (Wall. Overview of biological effects (pharmacology) of Myxopyrum smilacifolium (Wall) Blume.2 Overview of additional material for the production of instant tea. Stevia rebaudiana Bertoni.
Overview about extraction process. Overview of extraction solvents, extraction methods. Overview of extraction solvents. 16 iv Luan van 2.
Overview about instant tea. Soluble tea products .3 The expected production process for soluble tea from Myxopyrum smilacifolium Blume. Research contents and methods. Research object and research scope.
Place and time to proceed. Experimental design method. Data statistical analysis methods. Results and discussion.
The results of research results on the effect of ultrasonic treatment time on the efficiency of total polysaccharide from Myxopyrum smilacifolium Blume. The results of study on selecting solvent concentration to extract polysaccharides from Myxopyrum smilacifolium Blume. The results of selecting the ratio of raw materials Myxopyrum smilacifolium Blume/solvent. Research results of selecting the time to extract total polysaccharides from Myxopyrum smilacifolium Blume.
Research results of selecting temperature for extraction of total polysaccharides from Myxopyrum smilacifolium Blume. Research on choosing the suitable ratios of Myxopyrum smilacifolium Blume extract and additive ingredients. The results of the research on the drying temperature of mixture of Myxopyrum smilacifolium Blume extract and additive ingridients to produce instant tea. The complete process for producing instant tea from Myxopyrum smilacifolium Blume.
Conclusions and recommendation. 43 vi Luan van LIST OF TABLE Table 3. Experimental design to study the effect of ultrasonic treatment time on the extraction efficiency of polysaccharides in Myxopyrum smilacifolium Blume. Experimental design to select solvents concentration for extracting polysaccharides from Myxopyrum smilacifolium Blume.
Research on selecting the ratio of Myxopyrum smilacifolium Blume material with solvent. Experimental study on selecting the time of extraction. The effect of temperature of extraction on efficiency of polysaccharides extracted. The mixing ratio of Myxopyrum smilacifolium Blume extract and herbal additive extracts .10: Experimental arrangement to investigate the effect of drying temperature on instant tea sensory quality.
Instant tea weight coefficient for sensory evaluation. Scores of quality levels. Results about the effect of the ultrasonic treatment time on the efficiency of extraction total polysaccharide from Myxopyrum smilacifolium Blume 32 Table 4. The effect of the solvent concentration on the efficiency of extracting total polysaccharides from Myxopyrum smilacifolium Blume.
Effects of material/solvent ratio on efficiency of extracting total polysaccharides in Myxopyrum smilacifolium Blume. Effect of time on the efficiency of extracting total polysaccharides in Myxopyrum smilacifolium Blume. Effect of temperature on extraction efficiency of polysaccharides in Myxopyrum smilacifolium Blume. 35 vii Luan van Table 4.
The effects of ratios of Myxopyrum smilacifolium Blume extract and additive ingredients on the sensory quality of the product. The effects of drying temperature on sensory quality of the product. Ash and moisture contents of complete instant tea product. 43 viii Luan van LIST OF FIGUGES Figure 1.
Myxopyrum smilacifolium Blume. Structural formula of lactose. Stevia rebaudiana Bertoni. Instant tea products.
Diagram of instant tea production process. 39 ix Luan van LIST OF ABBREVIATIONS F Formula TCVN Vietnam standard x Luan van PART I. Research rationale Modern society leads to the increasing demand of people for quality of life, so many products are created to meet the needs of buyers. Tea is a cultural feature of Asia and has a very long tradition associated with history.
There are some studies that have found that tea originated from the Southwest China. It is a drink bearing the essence of Vietnam in particular and Asia in general, and is a cultural feature of each region. Tea used worldwide is also the second most popular beverage in the world after drinking water. Currently, in Vietnam as well as in the world, the trend of using medicinal plants of natural origin is increasing.
Drink tea properly to help quench your thirst as well as digestion, avoid drowsiness, stimulate kidney function, enhance eyesight, help clear mind, dispel drowsiness [38]. Tea products are increasingly diversified and diversified from quality and design. Besides the teas from the dried leaves, there are also tea bags and instant tea. In an era of development where time is not enough, the demand for beverages such as tea needs to be convenient and time-saving, instant tea is really essential, and soluble tea proves to be a lot of benefits.
It is beneficial for health and suitable for many customers and is extremely potential product for development. Since ancient times, plants belonging to ginseng group have been a precious herb, also known as a miracle herb, with a miraculous effect that ancient people recorded. Indeed, researchers have revealed many valuable pharmacial features of these plants such as enhancing vitality, fostering health, preventing disease, prolonging life. Currently there are many types of ginseng all over the world, based on the natural conditions of Vietnam and Myxopyrum smilacifolium Blume has been found almostly in the Northwest region of Viet Nam.
Myxopyrum smilacifolium Blume leaves have also been shown to contain compounds belonging to the group of alcaloid, carbohydrates, steroids, saponins, terpenoids, flavonoids, tannins and polyphenols [1], [2]. By blending some herbal extracts with Myxopyrum smilacifolium Blume extract, the soluble tea is obtained with many health benefits and extremely useful benefits, promoting economic development to create jobs for workers, making the tea market diversified , meeting the demand for health and natural products. Advantages of 1 Luan van Myxopyrum smilacifolium Blume are known to many people for its use and benefits. Based on discussion above, I conducted the project "Research on the process of producing instant tea from the Myxopyrum smilacifolium Blume plant".
Overall objective The overall objective of the research is to build the process of producing instant tea from Myxopyrum smilacifolium Blume plant, test production on a laboratory scale and supply to the market to create high quality tea products, bring economic efficiency, contribute to the diversification of tea products in the market and increase the application of Myxopyrum smilacifolium Blume plant. Detail objectives - Determining the influence of the suitable conditions for the following factors: time, temperature, solvent extracted, material/solvent ratio and concentration solvent on the amount of total polysaccharides of extraction; - Identifying the mixing ratio of Myxopyrum smilacifolium Blume and addtive ingredients to create instant tea; - Completing the process of producing soluble tea from Myxopyrum smilacifolium Blume extract. Scientific and practical significance 1. The scientific meaning of the topic - To provide more scientific information about the tree Myxopyrum smilacifolium Blume.
- To find out suitable parameters for the production process of instant tea from Myxopyrum smilacifolium Blume plant at laboratory scale. The practical meaning of the topic - To diversify products from Myxopyrum smilacifolium Blume to increase the value of this kind of herbs. s; - To create new products with good quality, good for the health of consumers and reasonable price; 2 Luan van - To create a new research direction on Myxopyrum smilacifolium Blume, and improve the output for this herbs. 3 Luan van PART II.
Overview about Myxopyrum smilacifolium (Wall. Characteristics of Myxopyrum smilacifolium (Wall.) Blume Myxopyrum smilacifolium Blume is a large woody twining shrub which is a plant of the genus Myxopyrum, the Jasmine family (Oleaceae). In addition, Myxopyrum smilacifolium Blume is also known as Nhuong Le Kim Cang which is a plant capable of growing through rocks and precious herb only found in old forests in the Northwest region of Viet Nam such as Ha Giang, Lao Cai, Lai Chau. where there is appropriate soil.
This plant lives mainly on the rock crevices of limestone mountains. The main stem and roots develop under the black humus soil caused by the decaying leaves of the Myxopyrum smilacifolium Blume tubers formed by the main trunk, each main stem section will form 2 - 4 roots with a length of 3 - 8cm. The genus Myxopyrum includes 4 species originally distributed in tropical and subtropical regions of East Asia. Its roots, stems, and leaves have a ton of medicinal effects and are used in many traditional medicine systems, especially in India.
The roots are used to treat diseases like scabies, cough, rheumatism, fever, and to treat wound healing. Leaves are astringent, sweet, thermogenic, used as an analgesic, allergy, cooling, and tonic. They are used in treating coughs, asthma, rheumatism, headache, fever, ear disease, and neuralgia [20]. Myxopyrum smilacifolium Blume.
Classification of the genus Myxopyrum [39] Domain: Eukaryota 4 Luan van Kingdom: Plantae Phylum: Tracheophita Class: Magnoliopsida Order: Lamiales Family: Oleaceae Genus: Myxopyrum Blume Species: Myxopyrum smilacifolium Blume The stem of Myxopyrum smilacifolium Blume is Shrub, four-sided low stem, rarely rounded. Leaves are simple, opposite, oval - broad oval, the entire margin of one or a half leaf, lateral veins 2-3 pairs stem from the leaf base, hairless, or with short, thin hairs. The panicle clusters on leaf axils, many flowers. Small flowers, bisexual, shaped cup, 4 lobes.
The color is yellow or pink, the tube is longer than the lobe or bell-shaped with the tube shorter than the lobe, 4 lobes, ovoid or broad, hairless. Secondly, flowers are attached near the root of the colon. Fruits have from 1 to 4 seeds [3]. The genus Myxopyrum is distributed in tropical and subtropical regions of Asia such as Bangladesh, Cambodia, India, Indonesia, Laos, Malaysia, Myanmar, New Guinea, Philippines, Thailand, China, Vietnam [21], [22], [23].
Distribution: There are 4 species in the world, distributed in Asian regions such as Bangladesh, Cambodia, India, Indonesia, Laos, Malaysia, Myanmar, New Guinea, Philippines, Thailand, China; There are 3 species in Vietnam: M.