VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY ------- ------- THESIS EVALUATE THE EFFECT OF HONEY AND CHOEROSPONDIAS AXILLARIS MIXTURE ON NECROTIC BACTERIA Hanoi, 2021 VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY ------- ------- THESIS TITLE EVALUATE THE EFFECT OF HONEY AND CHOEROSPONDIAS AXILLARIS MIXTURE ON NECROTIC BACTERIA Student : Bui Duc Hiep Faculty : Biotechnology Supervisors : Nguyen Thi Thuy Hanh, PhD. Hanoi, February 2021 COMMITMENT I hereby declare that: This is my study, which was conducted under the guidance of supervisors; All of information provided in this study is true and accurate All published data and information have been duly cited. Hanoi, February 2021 Student Bui Duc Hiep i ACKNOWLEDGEMENTS First, I am grateful to express my gratitude to the Thai Nguyen University of Medicne and Pharmacy for granting me support to pursue my thesis as well as the Department of Biotechnology, Vietnam National University of Agriculture, who provided me opportunities to pursue a higher education, anh to prepare myself to better. I also would like to thank my supervisor, Dr Nguyen Thi Thuy Hanh who has given me her encouragement, and help during my writing and revising process.
I owe a debt of gratitude to many people whose help has been important to my success in completing this dissertation. Firstly, I am deeply grateful to my major Bs. Nguyen Thi Hien and Mas. Nguyen Van Dung for their critical comments and useful suggestion.
Special for their help in collectiong data of my experiments. It would be difficult to mention everyone who has contributed to the research reported in this dissertation. I appreciate you all. It is also give my thankfulness to my family giving me various used advises during the process of learning and studying Thank you very much! Hanoi,February 2021 Student Bui Duc Hiep ii TABLE OF CONTENTS COMMITMENT.
ii TABLE OF CONTENTS.iii LIST OF TABLES. v LIST OF FIGURES. Evaluate the effect of Honey and Choerospondias axillaris mixture on necrotic bacteria. Purpose and Requirement.1 Introduction of Choerospondias axillaris .1 General introduction of Choerospondias axillaris .2 Botanical characteristic of Choerospondias axillaris .3 Necrosis of human skin .2 The main types of bacteria cause necrosis of human skin .3 The application of Choerospondias axillaris on folk drug.
15 III: MATERIALS AND METHODS .2 Time and place. Extraction of material from Choerospondias axillaris.Implementation of experiment In Vitro. Implementation of experiment on white mice .4 Experiments to assess the ability to kill bacteria Streptococcus and Pseudomonas aeruginosa cause skin necrosis of honey products and Choerospondias axillaris on white mice. RESULTS AND DISCUSSION .1 Extraction results for Choerospondias axillaris .2 Test in vitro .1 Research results on properties of Streptococcus of Honey and Choerospondias axillaris on a sterile ring .2 Research results on antibacterial properties of Pseudomonas aeruginosa of Honey product + Choerospondias axillaris extract on sterile ring.
Research results of the experiment determine the dose of bacteria that can cause necrosis on an individual mouse. Research results of the experiment evaluating the ability to kill Streptococcus and Pseudomonas aeruginosa cause skin necrosis of honey products - Choerospondias axillaris in white mice. CONCLUSION AND PROPOSAL. 36 iv LIST OF TABLES Table 3.
Mix with honey according to different ratios to get 5 formulas for studies .2 Evaluate the general situation of white mice before causing streptococus .3 Evaluate the general situation of mice before causing illness by the Pseudomonas aeruginosa .4 Assess the general situation of mice before causing disease .1 Results of bacterial inhibition loop on 3 experimental agar with Streptococcus. Variance and standard deviation of the formulas Resistant to Streptococcus. Anti-streptococcal effect of Preparations of Honey - Choerospondias axillaris. Results of bacterial inhibition loop on 3 experimental agar plates with Pseudomonas aeruginosa.
Variance and standard deviation of the formula resistant to Pseudomonas aeruginosa. Mix with honey according to different ratios to get 5 formulas for studies .2 Evaluate the general situation of white mice before causing streptococus .3 Evaluate the general situation of mice before causing illness by the Pseudomonas aeruginosa.4 Assess the general situation of mice before causing disease .1 Results of bacterial inhibition loop on 3 experimental agar with Streptococcus. Variance and standard deviation of the formulas Resistant to Streptococcus. Anti-streptococcal effect of Preparations of Honey - Choerospondias axillaris.
Results of bacterial inhibition loop on 3 experimental agar plates with Pseudomonas aeruginosa. Variance and standard deviation of the formula resistant to Pseudomonas aeruginosa25 vi LIST OF FIGURES Figure 2.1 Leaves and Fruits. Necrosis of the hand. Necrosis of the feet.
Medicinal herbs have been dried. Measure the diameter of the punching tool and the agar was perforated. Measure the bacterial inhibitory ring for the bacterial connective system. Direct antibacterial resistance of regime.
25 vii ABSTRACT Nowadays, the use of natural products to treat necrosis is of great interest to many scientists and pharmaceutical companies. The use of products derived from nature shows many advantages over the use of compounds and drugs that are synthesized by chemical methods. namely environmentally friendly, safe for users, low irritation rate, etc. However, besides that, it also has some limitations such as the need for an abundant source of raw materials, not much.
in-depth research works on those preparations. Most natural preparations used to treat necrosis are on the basis of folk remedies. Based on these advantages, thesis “Evaluate the effect of Honey and Choerospondias axillaris mixture on necrotic bacteria” was carried out to study and evaluate the effect in the treatment of necrosis disease for two main factors, streptococcus and Pseudomonas aeruginosa. The research results of the project show that the product from honey and choerospondias axillaris can treat necrosis in in vitro experiments and experiments performed on living individuals (white mice).
The medicinal use of the preparation is outstanding and is likely to be widely used in the future. Problem: In today's life, there are many dangerous diseases. Among the 15 most dangerous diseases, many lead to necrosis of the skin. Skin necrosis are necrotic ulcers that damage the most basic defense system, the patient's body is in pain and cannot resist the negative effects from outside, so ulcers can cause blood infection.
threatens the patient's life. The main cause of necrosis is a decrease in the blood supply to damaged tissues and cell death. [6] There are many different causes of skin necrosis, which can be named to the most basic ones, which are: Necrotic skin ulcers due to occlusion of the arteries; Peripheral neurological complications of diabetes; Cerebral vascular accident patient with human paralysis complications; Infected boils cause necrotic skin ulcers. To date, there have been a number of domestic and foreign studies on necrosis problem and necrosis treatment methods in different parts of the human body.
For example, the authors of the Hanoi Medical University Hospital conducted a study since 2012 on necrotizing fasciitis (necrotizing fasciitis). This is a bacterial infection usually caused by streptococcus or staph. Research has concluded: skin necrosis, muscle weight due to infection is a rare disease. High mortality if not treated promptly.
Combining medical treatment against infection, cleaning lesions, and skin patching is a treatment with good results. Evaluate the effect of Honey and Choerospondias axillaris mixture on necrotic bacteria Regarding the herbal necrosis treatment drugs, I had access to information about Nacurgo products containing Polyesteramide biofilm, Nano Curcumin and Camellia Sinensis green tea extract to help disinfect the stain. skin ulcers, allowing skin ulcers to heal 3 to 5 times faster than normal and do not lead to serious consequences such as sepsis leading to necrosis. [11] Traditionally, honey or products from choerospondias axillaris are used as support for the treatment of necrosis.
In fact, there is also no research and 1 evaluation on the combination of honey with choerospondias axillaris in bactericidal and necrotic treatment. Therefore, our group conducted research project to evaluate the bactericidal ability with honey and polymers. This research is conducted to provide more scientific evidence on the treatment of necrosis of honey and extracts of choerospondias axillaris, contributing to opening a new direction in research on drug formulation and Treatment for necrotic patients from the very plants and animals available in Vietnam. Purpose and Requirement 1.1 Purpose Provides scientific evidence of a folk remedy using choerospondias axillaris extractum in the treatment of necrotic ulcers caused by infections.2 Requirement - Extraction of material from Choerospondias axillaris - Implementation of experiment In Vitro - Implementation of experiment on white mice 2 II.1 Introduction of Choerospondias axillaris 2.1 General introduction of Choerospondias axillaris Scientific name: Choerospondias axillaris (Roxb.) Burtt et Hill (Spondias axillaris Roxb.), Belongs to the Anacardiaceae.
Other names: Oval tea, wood, forest oval, sliced oval, toads, male acid apple. Scientific classification Kingdom: Plantae Order: Sapindales Family: Anacardiaceae Genus: Choerospondias Species: C. Distribution Meliaceae (Meliaceae family) is a family of plants mainly tree trunks with about 50 varieties, 550 species distributed throughout the tropics. In the world, this plant is distributed in South Asian countries such as India, Nepal .; Southeast Asian countries such as Thailand, Malaysia, Indonesia, Laos, Cambodia and some southern provinces of China such as Guangdong, Hubei, Hunan, Guangdong, Guangxi, Yunnan, Fujian.
In Vietnam, Choerospondias axillaris is quite popular in most provinces across the country. Provinces with many Choerospondias axillaris include: Ha Giang, Tuyen Quang, Yen Bai, Lao Cai, Lang Son, Vinh Phuc, Ha Tay, Ninh Binh, Quang Tri, Dak Lak, Gia Lai, Kon Tum. and especially in Thai Nguyen. In the midland and mountainous areas in general and Thai Nguyen province in particular, the oval tree grows popularly due to favorable climatic and soil conditions: it is a tropical climate with rainfall over 1500mm / year and seasonal distribution; Slope topography is gentle, easy to drain with soils still forested in nature, moist, less acidic, deep and thick.
Chemical composition Chemical studies show that the oval family contains triterpenoid tirucallan, dramaran, oleanan, multifloran and limonoid. In addition, the rhizome bark and rhizome contain a bitter alkaloid (formula C9H8O4) that has a worm-killing effect. Furthermore, the fruit contains an alkaloid called azaridin and an oil (60%), the oil has sulfur and the smell of garlic. The leaves contain an alkaloid called paraisin, a small amount of rutin (0.
In 1975, Nguyen Liem and his colleagues analyzed the mollusc bark and found that there were 37.6% quirions, and 14% natural polymers. In particular, in the bark and root bark of the oval tree, it still contains 70% tannin (13.7% in stem bark; 1% in stem wood; 44.8% in root bark and 1.9% in root wood; , 5%); Flavonoids; Quinon; Plastic gum; Fatty oil. In the leaf bark about 13- 14% gum gum. [3] To serve the purposes of the topic, from the above chemical components of the oval tree, we pay special attention to the following three compounds: tannin, flavonoid and quinon.
The reasons the above compounds play an important role in research are: Tannins: Tannins are natural compounds in the polyphenol group common in plants. They are acrid and have tanning properties. This means being able to bind to the proteins of the skin to form a structure that is stable to the decay process. The molecular weight of tannin is about 1,000 - 5,000.
(The term "tannin" is used in the biological and food industries to refer to all natural polyphenols that have an acrid taste, but not these substances have a true tanning ability. This property is only available to compounds. High molecular weight molecules from 1,000 - 5,000. The molecules with lower molecular weight have only acrid taste without tanning properties, to distinguish it is called "food tannin", "tea tannin" .) In the plant, tannin is involved in metabolism, redox processes.
As polyphenols, tannins have antibacterial properties, so they play a protective role for 4 the plant. In particular, tannin solution combines with protein, forming a membrane on the mucosa, so it is used as a tonic.