THE JOINT ACADEMIC PROGRAM OF EXECUTIVE MASTER IN SCIENCES AND MANAGEMENT OF THE ENVIRONMENT BETWEEN INDUSTRIAL UNIVERSITY OF HOCHIMINH CITY AND LIEGE UNIVERSITY là or fe Université éX\'H:::::. yf INDUSTRIAL TA DUC TRUNG PRODUCTION OF ANTIBACTERIAL GLASS USING NANO SILVER Major: EXECUTIVE MASTER IN SCIENCES AND MANAGEMENT OF THE ENVIRONMENT MASTER’S THESIS HOCHIMINH CITY, 2022 The project was completed at The Industrial University of Hochiminh City Supervisor’s name: Associate Prof. Le Hung Anh (Write full name and signature) The thesis was taken at The Industrial University of Hochiminh City date 17 February, 2023. Nguyen Thanh Binh.
Nguyen Thi Thanh Truc. Nguyen Thi Ngoe.-------‹- - Secretary (Write full name and signature) COMMITTEE CHAIR DEAN OF INSTITUTE OF ENVIRONMENTAL > ho SCIENCE, ENGINEERING AND MANAGEMENT P0$. LÊ HÙNG ANH ACKNOWLEDGEMENTS In order to complete this graduation thesis report, I have received a lot of help, guidance and input from many people. First of all, | would like to express my sincere thanks to my instructor - lecturer of Ho Chi Minh City University of Industry - Assoc.
Le Hung Anh and Dr. Pham Trung Kien - lecturer at Ho Chi Minh City University of Technology, during the past time, he has guided me very enthusiastically. Thanks to the guidance of the teacher, I know how to study, work and research topics scientifically and effectively. And thanks to the dedicated guidance and guidance of the teacher, I have gained a lot of knowledge, so that I can complete this graduation thesis report.
Sincere thanks to the laboratory staff, of the Institute of Science, Technology and Environment Management and the Faculty of Materials Technology for creating the best conditions for equipment and tools during the experiment period. At the same time, I would like to express my thanks to the previous classmates of the environment, the wedsite management boards for documents on the environment. for creating conditions for the team to learn and investigate and survey to get the completed data project plan. Although I have tried to complete the report as much as possible, it inevitably has shortcomings, I hope to receive valuable contributions from teachers and professors.
ABTRACT Nanosilver (AgNPs) has good bactericidal ability to create a clean environment, minimizing the possibility of spreading diseases from glass and porcelain surfaces. The subject covered the nano silver layer on the glass surface by dipping and spinning coating technique. Through the methods: FT-IR (assessment of physical and chemical characteristics of solution), thereby determining coconut oil is a good solvent, and the ratio of resinate and coconut oil 1:20 (with 1 gram of solid resinate: 20 ml of coconut oil) selected as the optimal ratio to cover silver resintae with glass. XRD (identifying the product to be nano silver), SEM (determining the size of the particle after calcination), EDX (determining the composition of glass material after calcination with Ag + ions), UV-VIS (measuring the transmittance of materials after silver nanoparticles) a defined topic on the glass surface exists a layer of nano silver, the size of the nanoparticles is relatively evenly distributed on the surface of coated glass and the glass sample after coating has UV resistance is quite good.
In addition, the antibacterial test with five types of bacteria causing hospital infections is Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella Typhimurium, Bacillus cereus, the silver-coated glass for silver has very good results. All samples influenced the growth of bacteria, with the two types of Escherichia coli, Pseudomonas aeruginosa giving complete resistance. Since then, the thesis has had high applicability to reality and is the foundation for some economic sectors in general and the hospital environment in particular. TABLE OF CONTENTS LIST OF TABLE LIST OF FIGURES.
LIST OF ABBREVIATIONS CHAPTER 1: INTRODUCTION 1. The necessity of the topic 1. OF the SHUG: «sind aiustadharentycrenyacsnaciuemuncssnatssotoran alceuuyeen 2 1. Object ofresearch - Scope of research.ccocossoseccecsstex ccnporsiiissancusnesateneasasddvecneendvens 3 TG INOVELY SOE MNE TOPIC, 77.ctbseeaasteaneertzee sree Snrnnsinessndashbtanedan tro edranenenponcesdenstnsbecttoet 4 CHAPTER: 2.
OVER VIEW cississssscesscssoscascssosescessnsssvassonssseasosssdsssessssassosesceasvacseenee 5 2.of silver nanomaterials .sccccsosssessoedesdseasa tivsusncatvasescssooatoucadstepel 5 DAL e Silver Rano CONCE PE i siennciteesunces ovnder'davondldicaauleswnsssannnvedeensdeaeospancacsimeeataetetes See 5 DLO The chatactenisttesoF natiO SUV CR siaxssscssiondseesasbliG04sesc0sesisabksSs 6 2. Antiseptic mechanism of nano SilVer. Methods of synthesizing nano SỉÏV€T.-- +55 tre i 215s Application Of Nano SILVER.:0:coespecdeaneestnennenezeernenttonsaydenonetensebenneeentarstaia 10 D2 OVEVIEW OE COCONUE, OL a. tesssscrsccessiens sostssotenrcesdsticcuunscssealoosvenlsunlemedna venders 13 2.
General introduction about coconut-Oil cc ccreieveevsssseennsssencsesstenerenccice 13 2. Coconut oil production technology -.cssscesecslscciceeesceicstesceeqecseceneeseectnsereaces 14 2,263 ADIDIIGAHOI sysxsss1nsEitssesexrsinasasuepgliaslossssgrdkasxEd45EascuoSatisssaxslt U35 40aasu S5, Eg 17 D5: ME(hodS:0T:6LEHLTHESHIDINL se s32 ResisvasdlrerbeestasiorsthefmeraeaisiEetiiag ad tiệc 18 2. CHAPTER 3: MATERIALS AND METHODS 3. Materials and tools 3.
Lhe research meth Od ology. Determine the viscosity of a silver resinate mixture - eoconut oil. Determination of silver content in silver resinate mix(ure. Determined Dy FU UR thet 6d scssesssxssvosasossa ea cariariassseieneeeciell nauseanasd eanernencbons 39 4.
Determined by XRD method: s. Determination of silver size and elemental composition in coated and calcined ÏHSS, SAHIDIBRssriecsissssE llslobs2i01200x00p0g315606gasss,xes10x4stsa2aoVsadyoiasÐe th 42 431-:12eterrnninetl/Dy 2K) HTIEKHODseusicieictosarosaaaoslnoglisssasosasiaOcdubni 42 4. Observe the surface of SEM gÌaSs. Analysis of elemental composition of EDX glass.
Evaluate the results of silver nano coating on glass by UV - VIS method. Assess the antibacterial properties of nano-silver on representative bacteria TYPES ls scsstste a thestomenaegre cusssenauai es aM RUN Naceemecomu cats ccauce encores elanPa 45 4.sssscsssssssscassssiacscemnearasninanchiedarmbenieca 25a0i9386/x2xn s2 48 CHAPTER 5: CONCLUSIONS AND RECOMMENDATIONS. 50 (i6 ]HSTOHbuoi 220g 10aitlnplEt scS Dạ ti ỆG tho ae GD eseádi di AI 50 GHUOBExoroidsnsosaattiioedbdiiutonsbsdissytgpssaaimaasestidtlsdssislUnicusstUMAuraEai sltansui 51 TREEBERENCES oscycsossesovsenescavisossososvavecssvcusecussusrssvevivuvessbaivitvcivluvastsosesetessioneiaees 52 LIST OF TABLE Table 2. Number of silver atoms in a unit of volume.1: Chemicals used in research Table 3.2: Instruments used in the process of research.3: Equipment in the research process Table 4.1: Results of viscosity measurement Table 4.2: Antibacterial test results of glass (spin coating, dip coating).
LIST OF FIGURES Figure 2. silver element (Ag) Figure 2. Antiseptic mechanism of nano silver Figure 2.3: Application of silver nano in household products.4: Application of silver nano in agriculture Figure 2.5: Nano Silver water filter core.6: Application of silver nano in textiles Figure 2.7: Coconut essential oil $i Figure 2.9: Technology diagram of coconut oil production by hot pressing - hot Prescinorneth edn sce Wes cv nen ti 0 nhi 6m20 Liga tsatifs XE is f0 ues oceraie 16 Figure 2. Diagram of coconut oil production technology by centrifugal method SeucustassSocgbuvavslevstitaslnsuvscosctssiudscicedesass ova gevasetoavendes saul hevouds cuesbeucseacstsuecuals een taente ts 17 Figure 2.11: Principle of method of evaporation si LƠ Figure 2.12: Principle of sputtering process Figure 2.13: Evolution of Sol - gel process .14: Some methods of Sol - gel membrane coating Figure: 2.15: Dip = coating process.16: Steps of the Spin - coating process.1: Process diagram of manufacturing antibacterial glass.
Chart of experimental assessment 0Ÿ reSuÏfs .1: FT-IR spectrum of (a) NaOH 1M; (b) coconut oil, (c) AgNO3 and (d) silver resinate Figure 4.2: XRD result of Silver resinate sample after drying 105°C/18h a Figure 4.3: XRD result of Silver Resinate sample after heating 500°C / 3h.4: XRD spectrum of (a) uncoated glass sample; (b) sample of dip coated glass, calcined at 500°C/3 hours; and (c) Spinning coated glass sample, calcined at SOOLS/SEROUES ses fiers ae, Mies ent asset fe sssaedgunaen ieee maa rel eee 42 Figure 4.5: SEM images of dipped and spin coated glass samples at different magnifications 43 Figure 4.6: Results of EDX surface dip coated glasses.7: Results of EDX spin coating glass surfaC€s.8: Results of transmission oftwo ølass samples .----¿---+--<+ 45 LIST OF ABBREVIATIONS FT-IR Fourrier Transformation Insfrared X-XDR X-ray diffraction SEM Scanning Electron Microscope EDX Energy-dispersive X-ray spectroscopy UV-VIS Ultraviolet-Visible spectrophotometer vi CHAPTER 1: INTRODUCTION 1. The necessity of the topic Silver is the oldest known natural antibacterial agent. Silver and its compounds exhibit toxicity to bacteria, viruses, algae and fungi. Silver does not show toxicity to humans, non-toxic, non-irritant.
Scientific fact has proved that silver ions can destroy more than 650 strains of microorganisms, 260 types of harmful germs that cause disease and also gram-negative and positive bacteria. Silver inhibits the metabolism of respiration and the transport of substances through microbial cell membranes. Silver has the ability to destroy the enzyme that transports the nutrients of bacterial cells, weakens membranes, cell walls and cytoplasm, disrupts metabolism, leading to bacterial destruction. Silver Nano has the potential to be applied in many fields such as agriculture, industry and health to create many new and high quality products, bringing socio-economic efficiency.
The application of nano silver in health, life and manufacturing will also help protect human health, minimize environmental pollution and develop production. In medical use, silver nano solution is used: Covering artificial bone details, treating respiratory tract infections, treating urogenital infections, bandages for wounds such as burns, necrotic ulcers. From the advantages of nano silver as well as the usefulness, the difference of the method to create silver resinate from coconut oil is extremely necessary with the goal of going beyond creating an environmentally friendly product. Users can take advantage of available raw materials in Vietnam at low cost.
With the goal of creating a sterile environment for hospitals, and food processing facilities. researching silver nano-coated glass materials with coconut oil solvents. A type of organic substance very popular in Vietnam. Objectives of the study > General goal Development of silver nano coating materials to apply in practice, to improve the environment, prevent harmful bacteria to humans.
> Specific objectives Initial research on manufacturing antibacterial glass by coating nano silver with coconut oil solvents. Object of research - Scope of research 1. Research subjects - Nano silver 2 Glass - Coconut oil. Research scope > Space - To be accomplished with the support of the following laboratories: - Solid waste research and intensive research laboratory of the Institute of Science, Technology and Environment Management, Industrial University of Ho Chi Minh City.
- Silicate laboratory of Ho Chi Minh City University of Technology. - Center for BioScience and Biotechnology at VNUHCM-University of Science. - Glass: blue glass. - Solvents: coconut oil.
- Bacteria: Antibacterial test with 5 representative strains of bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella Typhimurium, Bacillus cereus). » Research period From December 2018 to August 2019. Research content - Manufacturing coconut oil soap and silver Resinate coating. - Coating nano silver on glass.
- Assessing antibacterial ability 1. Scientific and practical meanings » Scientific meaning: - Successful manufacture of silver resinate layer from coconut oil soap covering glass surface. - Researching on influencing technological factors on coating characteristics. >» Practical meaning: - The success of the project will certainly contribute to the development of high- quality coating materials for construction and civil glass products, especially for the health sector where glass materials are needed.
- The introduction of nano silver-coated glass with high bactericidal ability, will help improve public health. - In the future, this bactericidal glass material may be an alternative material such as smartphone screens, glasses. Novelty of the topic Current methods of nano silver coating are using expensive imported solvents. Therefore, we see the novelty of this topic in the use of coconut oil solvent is a natural material, popular in Vietnam and affordable.
Introduction of silver nanomaterials 2. Silver nano concept Silver nanoparticles are particles with a size of Inm silver to 100nm. Although often described as silver, some are made up of a large percentage of silver oxides due to the large proportion of surface silver atoms compared to their large number. Many shapes of commonly used nanoparticles, such as spherical, octagonal, and thin silver nanoparticles are also very common.
[33] Electron configuration of silver: 1872s72p°3s73p°3d! 4574p %4a! 5g! + Atomic radius Ag: 0,288 nm + Atomic radius silver ion: 0,23 nm Table 2. Number of silver atoms in a unit of volume Size of nanoparticles Ag | Number of atoms in it (am) 1 31 5 3900 20 250000 Figure 2. The characteristics of nano silver Nano silver material has a surface area greater than bulk materials so it can release more Ag+ ions, so silver nanoparticles have better antibacterial properties. Silver nanoparticles have the phenomenon of surface Plasmon resonance.