VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY ------- ------- GRADUATIONN THESIS TITLE: RESEARCH ON EXTRACTION OF COLOR PIGMENTS AND ESSENTIAL OIL FROM ORGANIC MATERIALS FOR LIPSTICK TRIALS Hanoi, 3/2021 VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY ------- ------- GRADUATIONN THESIS TITLE: RESEARCH ON EXTRACTION OF COLOR PIGMENTS AND ESSENTIAL OIL FROM ORGANIC MATERIALS FOR LIPSTICK TRIALS Student : Ta Hong Ngoc Faculty : Biotechnology Supervisors : Nguyen Thi Thuy Hanh, PhD. Nguyen Thi Hien, PhD. Hanoi, 3/2021 COMMITMENT I hereby declare that: This is my study, which was conducted under the guidance of the supervisors; All data provided are true and accurate; All published data and information have been duly cited. Hanoi, January 24th 2021 Student Ta Hong Ngoc i ACKNOWLEDGEMENTS Firstly, I am grateful to express my gratitude to the Department of Biotechnology for granting me support to pursue my thesis, Vietnam National University of Agriculture, who provided me opportunities to pursue a higher education, and to prepare myself to better.
I would like to express my deep gratitude to the teacher, PhD. Nguyễn Thị Thúy Hạnh – Factulty of Biotechnologi in Viet Nam National University of Agriculture, who guided me through all the time working on this topic and completing this topic. I owe a debt of gratitude to sincerely thank PhD. Nguyễn Thị Hiển – Lecturer of Faculty of Enviroment in Viet Nam National University of Agriculture for suggesting, guiding me very wholeheartedly during my time working on the topic.
This is also an opportunity for me to have the opportunity to thank my family and friends, who are always there to help, support and encourage me during the process of training, studying and researching for me to complete this subject. Thank you very much! Hanoi, January 24th 2021 Student Ta Hong Ngoc ii TABLE OF CONTENTS COMMITMENT. ii TABLE OF CONTENTS. iii LIST OF TABLES.
vi LIST OF FIGURES. Basic information about researching material. Red dragon fruit: [4]. General introduction of red dragon fruit.
Analysis of ingredients. General introduction of gac fruit. Analysis of ingredients. General introduction of tumeric.
Analysis of ingredients. General introduction of pomelo. Analysis of ingredients. Organic materials compound lipstick base.
Cosmetic trendy nowaday. Application of researching materials.13 iii CHAPTER III. MATERIALS AND METHODS. Materials, contents and method.
Time and place. Experiments of extraction from fruit and flower. Extracting pink pigment from red dragon fruit. Extracting yellow pigment from tumeric.
Extracting red pigment from gac fruit. Extracting essential oil from pomelo flower. Experiments combining organic material and extractions making lipstick. Making lipstick base by organic material.
Finding out the best formulation. Application of pigment extractions on coloring lipstick. Application of essential oil extractions on flavouring lipstick. RESULTS AND DISCUSSION.
Extraction result of pigment from red dragon. Extraction result of pigment from tumeric. Extraction result of pigment from gac. Extraction result of pomelo flowers.
Trial combining material and extrction making lipstick results. Result of using organic materials making lipstick base. Result of finding out the best formualation of lipstick base. Result of application of pigment extractions on coloring lipstick.
CONCLUSION AND PROPOSAL .36 v LIST OF TABLES Table 3. Experimental arrangement of color extraction from red dragon fruit. Experimental arrangement of color extraction from tumeric. Experimental arrangement of color extraction from Gac.
Experimental arrangement of essential oil extraction from pomelo flower. Experiment evaluate the smooth and hard or soft of lipstick base. Experiment evaluate the storage condition of lipstick base. A mount of yellow pigment adding into lipstick base.
A mount of strong pink pigment adding into lipstick base. A mount of red pigment adding into lipstick base. A mount of essential oil adding into lipstick base .1 Extraction result of pigment from red dragon fruit .2 Extraction result of pigment from tumeric .3 Extraction result of pigment from Gac .4 Extraction result of pomelo flower .5 Evaluation result of character of lipstick base .6 Evaluation result of storage condition of fomualtion 4 and 5 .7 Coloring lipstick results with yellow pigment (cucurminoid) .8 Coloring lipstick results with strong pink pigment (betacyanin) .9 Coloring lipstick results with red pigment (lycopene and beta carotene) .10 Flavouring lipstick results with essential oil from pomelo flower .33 vi LIST OF FIGURES Figure 2.1 Red dragon fruit. Purified soybean wax.
Tumeric powder stored in box. Dried gac powder. Pigment obtained from Gac. Weight beewax and soybean wax.
Mixture after adding oil. Adding pigment into mixture of lipstick base. Pigment obtained from red dragon fruit. Color pigment obtained from tumeric.
Color pigment obtained from gac. Essential oil obtained from pomelo flower .30 vii ABSTRACT Nowadays, using compounds extracted from organic ingredients in daily life as well as in functional foods and cosmetics is a lot of interest from scientists as well as companies. The use of plant-derived compounds shows many advantages over the use of compounds synthesized by chemical methods, such as: environmentally friendly, safe for users, low allergy rate, abundant raw materials… However, it also has some limitations such as: raw materials need to be ensured in terms of biosafety, technology of extracting is so complex,…. Based on such need and urgency, the thesis “Research on extraction of color pigments and essential oil from organic materials for lipstick trials” was carried out, research and use natural ingredients to replace toxic ingredients in lipsticks.
Result of reseaching show that, we can use the extraction from fruit such as red dragon fruit, tumeric, gac and some oraganic materials to replace the toxic ingredients in industrial lipstick. Beside the characters of them still are the same industrial lipstick’s. INTRODUCTION The statistics show that the number of people using cosmetics is extremely large, not just women. One of the most used products is lipstick.
According to one survey, on average, each woman uses up 20kg of lipstick in her lifetime and they swallow 2kg of it. [1] The aforementioned toxic ingredients are actually potentially hazardous. Recently, the US Drug Administration (FDA) studied the composition of 400 other lipsticks, the results published showed that lead content in the lipsticks tested increased from 0,026 -7,19ppm, compared with the average of 1. The content of lead in lipstick is increasing a lot.
[1] However, the FDA still announced on its official website that this lead content is still within safe limits and there is no evidence that it can harm the health of consumers. FDA has also proven that in addition to lead, in lipstick also contains other chemical compounds such as triclosan, cadmium, mathacrylate, parabens, mirical oil. which, according to research, triclosan substances greatly affect the activity of the heart muscle and can Female infertility potential. Toxins in lipstick not only cause dermatitis, rashes, and have a great impact on the heart muscle activity, female reproductive function, even causing infertility in women.
It can not be confirmed immediately that it is harmless to health. Long-term lipstick users are most likely to suffer from skin diseases such as dermatitis, atopic skin ., a group of experts in the US has also demonstrated that in chemical compounds such as triclosan, cadmium, lead, mathacrylate, parabens. In lipstick, triclosan has a great effect on the function of the heart muscle and is capable of causing infertility in women. Regular use or exposure to triclosan will be one of the causes of infertility in women.
Women who use lipstick too early cause the body to absorb triclosan for quite a long time will significantly increase the production of male hormone levels in the female body, leading to disorders of the menstrual cycle to be a mother. Only a quick study, we can also see harmful ingredients and possible consequences for the health of consumers. Although on the market today there are many lip balms, unleaded lipsticks, lipsticks containing natural ingredients. but in general, these 1 lipsticks still need to use artificial colorants, ie still use chemical composition.
So how to meet the increasing beauty needs of women and limit the negative consequences and harmful effects of using lipstick? Based on such need and urgency, the research team would like to propose research and use natural ingredients to make safe lipsticks. We already know a lot of lipsticks with natural ingredients on the market, but this topic focuses on natural ingredients that will be the main ingredients, largely replacing unhealthy chemical ingredients. Specifically, the lipstick materials will be beeswax, olive oil, vitamins, coconut oil. especially the lipstick color will be obtained from natural products.
With the thesis “Research on extraction of color pigments and essential oil from organic materials for lipstick trials” 1. Purpose Using the extraction from some organic material for making organic lipstick, replacing toxic pigment, toxic flavour in industrial lipstick by pigment, essential oil from extractions of fruits. Extraction of color pigments: + Extraction of yellow pigment from tumeric. Extraction of strong pink pigment from red dragon fruit.
Extraction of red pigment from gac fruit. Extraction of essential oil from pomelo. Trial on mixing organic material and extractions for making lipstick. Basic information about researching material.
Red dragon fruit: [4] 2. General introduction of red dragon fruit. Scientific name: Hylocereus costaricensis. Distribution: This is a native plant species in Mexico, Central America and South America.
Currently, these species are also grown in countries in Southeast Asia such as Vietnam, Malaysia, Thailand, Philippines, Indonesia (especially in the west of Java Island); southern China, Taiwan and some other regions. Analysis of ingredients Analysis of ingredients: Red dragon fruit contains lycopene, betacyanin (the main pigment), a natural antioxidant that can fight cancer, heart disease and lower blood pressure. This is a very beautiful and healthy fruit. Red flesh dragon fruit has completely different properties compared to conventional white dragon fruits on the market today.
Outside red dragon fruit has a hard shell, bright dark red color; the inside is crimson red like lipstick, fancy, it becomes twice as nutritious powder as white dragon fruit. With the indexes of Vitamin C 12 - 6, Protid 1,30 - 1.08, Vitamin A, Glucid, Lipit, red dragon fruit brings very high nutritional value. Red flesh dragon fruit is a new, strange, delicious, nutritious product, a "made in Vietnam" product. Currently, the United States has issued an export code for red flesh dragon fruit and with advanced production techniques, red flesh dragon fruit is a product that strictly complies with food safety, pesticide residue as well as some other requirements.
has been trusted by export partners from the US, Japan, and Europe. 3 The characteristic of this dragon fruit is that it looks very small but weighs (~ 1kg / 1 fruit), bright red flesh, crispy, fragrant rice, especially sweet, lots of vitamins and minerals. Although the price of this fruit is slightly higher than that of white flesh dragon fruit, it has a particularly delicious taste, and becomes a high-class dish that is both delicious and strange to domestic and foreign consumers. According to a scientific document (scientific journal) recently published, the active ingredient Lycopene has anti- cancer and anti-aging effects.
found in red fruits such as Gac, Carot. and especially is red flesh dragon fruit. In addition, according to Mr. Phung Nhat Phong, owner of the pilot production and processing facility and the Figure 2.1 Red dragon fruit first farmer to pilot red-flesh dragon fruits in Tay (Source: Internet) Ninh, said: “When eating, the fruit leaves the red color on Natural lips are very beautiful.
The fruit has a very high natural color content, can process food colors, produce cosmetics such as lipstick, make-up powder, can treat cancer, make wine good for the health of the elderly and women (the Institute Paster acknowledged). In addition to normal use, we can squeeze dragon fruit juice to drink very cool. In the process of pressing the broken seeds, providing more fiber good for digestion, helping to laxative. General introduction of gac fruit.
Scientific name: Momordica cochinchinensis.