THE JOINT ACADEMIC PROGRAM OF EXECUTIVE MASTER IN SCIENCES AND MANAGEMENT OF THE ENVIRONMENT BETWEEN INDUSTRIAL UNIVERSITY OF HOCHIMINH CITY AND LIÈGE UNIVERSITY LIÈGE Xi.H INDUSTRIAL Université UNIVERSITY OF HOCHIMIMH CITY TA DUC TRUNG PRODUCTION OF ANTIBACTERIAL GLASS USING NANO SILVER Major: EXECUTIVE MASTER IN SCIENCES AND MANAGEMENT OF THE ENVIRONMENT MASTER’S THESIS HOCHIMINH CITY, 2022 The project was completed at The Industrial University of Hochiminh City Supervisor’s name: Associate Prof. Le Hung Anh. (Write full name and signature) The thesis was taken at The Industrial University of Hochiminh City date 17 February, 2023. Nguyen Thanh Binh.
Nguyen Thi Thanh True. Nguyen Thi Ngoc. - Secretary (Write full name and signature) COMMITTEE CHAIR DEAN OF INSTITUTE OF ENVIRONMENTAL SCIENCE, ENGINEERING AND MANAGEMENT POS TS. LÊ HỪNG ANH ACKNOWLEDGEMENTS In order to complete this graduation thesis report, I have received a lot of help, guidance and input from many people.
First of all, I would like to express my sincere thanks to my instructor - lecturer ofHo Chi Minh City University of Industry - Assoc. Le Hung Anh and Dr. Pham Trung Kien - lecturer at Ho Chi Minh City University of Technology, during the past time, he has guided me very enthusiastically. Thanks to the guidance of the teacher, I know how to study, work and research topics scientifically and effectively.
And thanks to the dedicated guidance and guidance of the teacher, I have gained a lot of knowledge, so that I can complete this graduation thesis report. Sincere thanks to the laboratory staff, of the Institute of Science, Technology and Environment Management and the Faculty of Materials Technology for creating the best conditions for equipment and tools during the experiment period. At the same time, I would like to express my thanks to the previous classmates of the environment, the wedsite management boards for documents on the environment. for creating conditions for the team to learn and investigate and survey to get the completed data project plan.
Although I have tried to complete the report as much as possible, it inevitably has shortcomings, I hope to receive valuable contributions from teachers and professors. i ABTRACT Nanosilver (AgNPs) has good bactericidal ability to create a clean environment, minimizing the possibility of spreading diseases from glass and porcelain surfaces. The subject covered the nano silver layer on the glass surface by dipping and spinning coating technique. Through the methods: FT-IR (assessment of physical and chemical characteristics of solution), thereby determining coconut oil is a good solvent, and the ratio of resinate and coconut oil 1:20 (with 1 gram of solid resinate: 20 ml of coconut oil) selected as the optimal ratio to cover silver resintae with glass.
XRD (identifying the product to be nano silver), SEM (determining the size of the particle after calcination), EDX (determining the composition of glass material after calcination with Ag + ions), ƯV-VIS (measuring the transmittance of materials after silver nanoparticles) a defined topic on the glass surface exists a layer of nano silver, the size of the nanoparticles is relatively evenly distributed on the surface of coated glass and the glass sample after coating has uv resistance is quite good. In addition, the antibacterial test with five types of bacteria causing hospital infections is Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella Typhimurium, Bacillus cereus, the silver-coated glass for silver has very good results. All samples influenced the growth of bacteria, with the two types of Escherichia coli. Pseudomonas aeruginosa giving complete resistance.
Since then, the thesis has had high applicability to reality and is the foundation for some economic sectors in general and the hospital environment in particular. ii TABLE OF CONTENTS LIST OF TABLE. V LIST OF FIGURES. V LIST OF ABBREVIATIONS.
vi CHAPTER 1: INTRODUCTION. The necessity of the topic. Objectives of the study. Object of research - Scope of research.
Scientific and practical meanings. Novelty of the topic. Introduction of silver nanomaterials. Silver nano concept.
The characteristics of nano silver. Antiseptic mechanism of nano silver. Methods of synthesizing nano silver. Application of nano silver.
Overview of coconut oil. General introduction about coconut oil. Coconut oil production technology. Methods of creating films.
21 CHAPTER 3: MATERIALS AND METHODS. Materials and tools. The research methodology. 31 iii CHAPTER 4: RESULTS AND DISCUSSION.
Determine the viscosity of a silver resinate mixture - coconut oil. Determination of silver content in silver resinate mixture. Determined by FTIR method. Determined by XRD method.
Determination of silver size and elemental composition in coated and calcined glass samples. Determined by XRD method. Observe the surface of SEM glass. Analysis of elemental composition of EDX glass.
Evaluate the results of silver nano coatingon glass by uv - VIS method. Assess the antibacterial properties of nano-silver on representative bacteria types.48 CHAPTER 5: CONCLUSIONS AND RECOMMENDATIONS. 52 iv LIST OF TABLE Table 2. Number of silver atoms in a unit of volume.1: Chemicals used in research.2: Instruments used in the process of research.3: Equipment in the research process.1: Results of viscosity measurement.2: Antibacterial test results of glass (spin coating, dip coating).46 LIST OF FIGURES Figure 2.
Antiseptic mechanism of nano silver.3: Application of silver nano in household products.4: Application of silver nano in agriculture.5: Nano Silver water filter core.6: Application of silver nano in textiles.7: Coconut essential oil.9: Technology diagram of coconut oil production by hot pressing - hot pressing method. Diagram of coconut oil production technology by centrifugal method .11: Principle of method of evaporation.12: Principle of sputtering process.13: Evolution of Sol - gel process.14: Some methods of Sol - gel membrane coating.15: Dip - coating process.16: Steps of the Spin - coating process.1: Process diagram of manufacturing antibacterial glass. Chart of experimental assessment of results.1: FT-IR spectrum of (a) NaOH IM; (b) coconut oil, (c) AgNO3 and (d) silver resinate.2: XRD result of Silver resinate sample after drying 105°C/18h.3: XRD result of Silver Resinate sample after heating 500°C / 3h.4: XRD spectrum of (a) uncoated glass sample; (b) sample of dip coated glass, calcined at 500°C/3 hours; and (c) Spinning coated glass sample, calcined at 500oC/3 hours.5: SEM images of dipped and spin coated glass samples at different magnifications.6: Results of EDX surface dip coated glasses.7: Results of EDX spin coating glass surfaces.8: Results of transmission of two glass samples. 45 LIST OF ABBREVIATIONS FT-IR Fourrier Transformation Insfrared X-XDR X-ray diffraction SEM Scanning Electron Microscope EDX Energy-dispersive X-ray spectroscopy UV-VIS Ultraviolet-Visible spectrophotometer vi CHAPTER 1: INTRODUCTION 1.
The necessity of the topic Silver is the oldest known natural antibacterial agent. Silver and its compounds exhibit toxicity to bacteria, viruses, algae and fungi. Silver does not show toxicity to humans, non-toxic, non-irritant. Scientific fact has proved that silver ions can destroy more than 650 strains of microorganisms, 260 types of harmful germs that cause disease and also gram-negative and positive bacteria.
Silver inhibits the metabolism of respiration and the transport of substances through microbial cell membranes. Silver has the ability to destroy the enzyme that transports the nutrients of bacterial cells, weakens membranes, cell walls and cytoplasm, disrupts metabolism, leading to bacterial destruction. Silver Nano has the potential to be applied in many fields such as agriculture, industry and health to create many new and high quality products, bringing socio-economic efficiency. The application of nano silver in health, life and manufacturing will also help protect human health, minimize environmental pollution and develop production.
In medical use, silver nano solution is used: Covering artificial bone details, treating respiratory tract infections, treating urogenital infections, bandages for wounds such as bums, necrotic ulcers. From the advantages of nano silver as well as the usefulness, the difference of the method to create silver resinate from coconut oil is extremely necessary with the goal of going beyond creating an environmentally friendly product. Users can take advantage of available raw materials in Vietnam at low cost. With the goal of creating a sterile environment for hospitals, and food processing facilities.
researching silver nano-coated glass materials with coconut oil solvents. A type of organic substance very popular in Vietnam. Objectives of the study > General goal Development of silver nano coating materials to apply in practice, to improve the environment, prevent harmful bacteria to humans. > Specific objectives Initial research on manufacturing antibacterial glass by coating nano silver with coconut oil solvents.
Object of research - Scope of research 1. Research subjects Nano silver Glass Coconut oil. Research scope > Space - To be accomplished with the support of the following laboratories: - Solid waste research and intensive research laboratory of the Institute of Science, Technology and Environment Management, Industrial University of Ho Chi Minh City. - Silicate laboratory of Ho Chi Minh City University of Technology.
- Center for BioScience and Biotechnology at VNUHCM-University of Science. 2 - Glass: blue glass. - Solvents: coconut oil. - Bacteria: Antibacterial test with 5 representative strains of bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella Typhimurium, Bacillus cereus).
> Research period From December 2018 to August 2019. Research content - Manufacturing coconut oil soap and silver Resinate coating. - Coating nano silver on glass. - Assessing antibacterial ability 1.
Scientific and practical meanings > Scientific meaning: - Successful manufacture of silver resinate layer from coconut oil soap covering glass surface. - Researching on influencing technological factors on coating characteristics. > Practical meaning: - The success of the project will certainly contribute to the development of high- quality coating materials for construction and civil glass products, especially for the health sector where glass materials are needed. - The introduction of nano silver-coated glass with high bactericidal ability, will help improve public health.
- In the future, this bactericidal glass material may be an alternative material such as smartphone screens, glasses. Novelty of the topic Current methods of nano silver coating are using expensive imported solvents. Therefore, we see the novelty of this topic in the use of coconut oil solvent is a natural material, popular in Vietnam and affordable. Introduction of silver nanomaterials 2.
Silver nano concept Silver nanoparticles are particles with a size of Inm silver to lOOnm. Although often described as silver, some are made up of a large percentage of silver oxides due to the large proportion of surface silver atoms compared to theữ large number. Many shapes of commonly used nanoparticles, such as spherical, octagonal, and thin silver nanoparticles are also very common. [33] Electron configuration of silver: ls22s22p63s23p63d104s24p64d105s1 + Atomic radius Ag: 0,288 nm + Atomic radius silver ion: 0,23 nm Table 2.
Number of silver atoms in a unit of volume Size of nanoparticles Ag Number of atoms in it (nm) 1 31 5 3900 20 250000 Figure 2. The characteristics of nano silver Nano silver material has a surface area greater than bulk materials so it can release more Ag+ ions, so silver nanoparticles have better antibacterial properties. Silver nanoparticles have the phenomenon of surface Plasmon resonance. This phenomenon makes the solution containing silver nanoparticles have different colors depending on the concentration and size of nanoparticles.
High chemical stability, not change under the effect of light and oxidizing agents - reducing the usual. Stability at high temperatures. Antiseptic mechanism of nano silver Antibacterial properties of nano-silver are explained by several mechanisms: Figure 2. Antiseptic mechanism of nano silver The first mechanism (Figure 2.2A): Inhibiting the transport process of Na+ and Ca2+ ions across the cell membrane, preventing metabolism.
The second mechanism (Figure 2.2B): Break down cell membranes, oxidizing the protoplasm of bacterial cells, protoplasm destroyed by oxygen dissolved in water with a catalytic role of Silver. 6 The third mechanism (Figure 2.2C): Indirect effects on DNA molecules by increasing the number of free radicals that reduce the activity of active oxy gen containing compounds, disrupt oxidation as well as phosphorylation in bacterial cells. The fourth mechanism (Figure 2.2D): Disabling enzyme containing -SH Sunphohydril group and - COOH, disrupting osmotic pressure equilibrium, or complexing with nucleic acids resulting in changes in the DNA structure of microbial cells, (direct impact on DNA structure).