THAI NGUYEN UNIVERSITY UNIVERSITY OF AGRICULTURE AND FORESTRY LE DUC CANH RESEARCH ON THE PRODUCTION PROCESS OF INSTANT TEA FROM ANGELICA SINENSIS UNIVERSITY GRADUATION COURSE Study mode: Full-time Major: Food technology Faculty: Advanced Education Program Office Supervisor: MSc. Dinh Thi Kim Hoa Batch: 2017-2022 Thai Nguyen, 13/5/2022 DOCUMENTATION PAGE WITH ABSTRACT Thai Nguyen University of Agriculture and Forestry Degree Program Bachelor of Food Technology Student name Canh Le Duc Student ID DTN1754290004 Thesis Title Research on the production process of instant tea from Angelica sinensis Supervisor (s) MSc.Dinh Thi Kim Hoa Supervisor’s Signature Abstract: Angelica sinensis ginseng is a type of ginseng with high nutritional and economic value grown and consumed in the northern mountainous provinces such as Lao Cai, Thai Nguyen, and Bac Can. This study focuses on the extraction of polysaccharides from Angelica sinensis ginseng and the development of a production process for instant tea. The study used an extraction method with liquid solvent to find the optimal extraction conditions for polysaccharides.
The results show that the optimal extraction conditions for Angelica sinensis ginseng include: the solvent used is Ethanol, the solvent concentration is 80o, the extraction time is 120 minutes in the thermostatic tank, and the ratio of material/ethanol is obtained using 1/15 and the extraction temperature was 70°C. The maximum content of polysaccharides achieved was 9. The experiment continued with the production process of instant tea from Angelica sinensis ginseng with the mixing ratio of 54.25g glucose, 45ml of Angelica sinensis ginseng extract, and 0.75ml of stevia extract, respectively. Instant tea powder was also evaluated for organoleptic and antioxidant activity.
The complete process is described in detail in the thesis. This is the basis for future studies on the extraction and production of instant tea from Angelica sinensis ginseng.Keywords: Angelica sinensis, Polysaccharides, Teabag, Extraction Number of pages: Date of Submission: i Acknowledgment To complete this thesis, I have received enthusiastic help and encouragement from individuals and groups. First, I would like to thank the teachers of the Department of Biotechnology- Food Technology, Thai Nguyen University of Agriculture and Forestry for creating all conditions to help me successfully complete this graduation thesis. I would like to acknowledge the dedicated dedication of Mr.
Dinh Thi Kim Hoa and Ms. Luu Hong Son, a lecturer at the Department of Biotechnology-Food Technology, Thai Nguyen University of Agriculture and Forestry, who did their best to help, give advice and useful knowledge so that I could complete my graduation thesis. I would like to express my sincere thanks to all the teachers who helped and created all conditions for me during my research project. I thank the Advanced Education Program Office and the administration of Thai Nguyen University of Agriculture and Forestry for providing detailed information and guidelines and helping me to complete my graduation thesis.
Thai Nguyen, May 13, 2022 Student Canh Le Duc ii Table contents DOCUMENTATION PAGE WITH ABSTRACT. iii Table of tables. 1 Table of Figures. Research questions and hypotheses.
5 PART 2: LITERATURE REVIEW. Chemical composition of Angelica sinensis. Organic Acids and Substances. Bioactivity and health effects of Angelica sinensis.
Anti-microbial effect. Anti-cancer effect. Anti-cardiovascular effects:. Anti-platelet effect.
Immune and hematopoietic support:. Extraction of Angelica sinensis. The processing technology of instant tea. 20 PART III METHODS .2 Place and time of research.
23 Content 1: Research on the chemical composition of Angelica sinensis. 23 Content 2: Research on the extraction process of Polysaccharides in Angelica sinensis. 23 Content 3: Research on creating instant tea products from total polysaccharide extracts from Angelica sinensis. Experimental set-up method.
Determination of moisture by drying to constant mass. Determination of ash content TCVN 8124: 2009. Quantitative method of total polysaccharide after extraction .4 Evaluation of antioxidant capacity. Data processing methods.
RESULTS AND DISCUSSION .1 Analytical results of the chemical composition of Angelica sinensis. Research results of the process of extracting Polysaccharide from Angelica sinensis.1 Research results on the types of solvents for extracting polysaccharides from Angelica sinensis. Research results on the concentration of solvents for extracting polysaccharides from Ginseng Angelica sinensis. Research results on the effect of treatment time with a water bath on the extraction efficiency of polysaccharides in Angelica Sinesis Ginseng.
Research results on a selection of the ratio of Angelica sinensis material/extraction solvent.5 Research results on a selection of extraction temperatures for compounds from Ginseng Angelica sinensis. Research results on formulating instant tea production. Research results on antioxidant activity. Research results on mixing ratio.3 Complete diagram of the production process of instant tea from Angelica sinensis.
Preliminary product cost calculation:. 69 v Table of tables Table 1: Research equipment. 21 Table 2: The selection of suitable solvent will improve the extraction efficiency of compounds. Conduct surveys on the following type of solvent: Water, ethanol, methanol.
24 Table 3: The selection of suitable solvent concentration will improve the extraction efficiency of compounds. Conduct surveys at the following concentrations: 60, 70, 80, 90, and 96% as follows:. 25 Table 4: Survey conducted at time points: 60, 90, 120, 150, 180. 26 Table 5: Experimenting with the ratio of raw materials/solvent according to 4 formulas: 1/10, 1/15, 1/20, 1/25.
26 Table 6: The extraction temperature strongly influences the extraction process and gives the energy and solvent costs. Conduct surveys at the following temperatures: 60, 70, 80, and 90 under the same conditions:. 27 Table 7: Extraction time strongly influences the extraction process on energy and solvent costs. Survey the following time levels: 30, 60, 90, and 120 minutes under the same conditions:.
28 Table 8: Mixing ratio between extracts from ginseng Angelica sinensis, glucose, and stevia. 31 Table 9: Sensory evaluation results will be evaluated through the Hedonic scale taste evaluation board by giving a score from 1 to 5 with the following points:. 31 Table 10: Development of a calibration curve using glucose. 34 Table 11: Results of total ash and moisture content.
36 Table 12: Effect of type solvents on the extraction efficiency of polysaccharides in Ginseng. 36 Table 13: Effect of ethanol solvent concentration on the extraction efficiency of polysaccharides in Ginseng. 38 Table 14: Effect of the treatment time of the water bath on the extraction efficiency of polysaccharides in Angelica sinensis Ginseng. 39 Table 15: Effect of ethanol/raw material ratio on polysaccharide extraction efficiency in Angelica sinensis Ginseng.
42 Table 16: Effect of temperature on the extraction efficiency of polysaccharides in Ginseng Angelica sinensis. 43 Table 17: Results of the sensory evaluation. 46 Table 18: Sensory evaluation scores for the criteria. 47 1 Table of Figures Figure 1:.
Angelica sinensis root (Credit: Ebay). 7 Figure 2: Ferulic acid. 9 Figure 3: Angelica sinensis. 21 Figure 4: The glucose standard graph.
34 Figure 5: Results of the type of solvents for extracting polysaccharides. 37 Figure 6: Results of the ethanol concentration for extracting polysaccharides. 39 Figure 7: Results of extraction time. 41 Figure 8: Results of material-slovent ratio.
42 Figure 9: Results of extraction temperature. 44 Figure 10: DPPH scavenging ability of Angelica sinensis. 45 Figure 11: Complete diagram of the production process of instant tea from Angelica sinensis. In today's modern life, synthetic drugs are very rich and diverse, with quick effects, and a short treatment time, but long-term use will cause many side effects affecting the health of patients.
Therefore, the use of natural medicines is becoming more and more popular. In Oriental medicine, Danggui has a sweet, spicy taste, has the effect of tonic blood, energizing blood, laxative, active field, regulating blood, and opening the meridian. Angelica sinensis is a very popular medicine, the first ingredient in the treatment of women's diseases, and also used in many other tonic and medical prescriptions. According to traditional medicine, Angelica sinensis is a very popular medicine in oriental medicine, the first ingredient in medicine for women, and is also indicated in many tonics and medical prescriptions such as for anemia and pain.
head, body emaciation, heart failure, fatigue, back pain, abdominal pain, arthritis, cold pain in the limbs, paralysis, paralysis, constipation, boils, itchy sores, hemorrhagic lesions, Irregular menstruation, menstrual pain, menstrual bleeding (taken 7 days before menstruation). Take 10-20g a day, in the form of a decoction or medicinal alcohol, Angelica sinensis is used to treat irregular menstruation, blood stasis, menstrual pain, menstrual cramps, uterine prolapse, bleeding, rheumatism, boils, constipation, baldness, anemia, tuberculosis, high blood pressure, cancer, and as a pain reliever, anticonvulsant, sweating, stimulating appetite. Women who drink Angelica sinensis decoction a few days before giving birth will ease labor and relieve pain during childbirth. According to modern medical research, Angelica sinensis contains many groups of precious active ingredients such as Essential oils, the most important of which is ligustilid, which increases blood circulation, and n-butylphtalid to treat stroke caused by an acute ischemic stroke.
count; immune-boosting and tumor-suppressing polysaccharides; coumarins have hemodynamic effects; phytoestrogens reduce the oxytocin-type effects of 3 pituitary hormones, inhibit uterine contractions, anti-inflammatory, and lower blood pressure; Ferulic acid has an inhibitory effect on platelet aggregation. Overall objective Overall objective: Developing high-tech scientific products from Angelica sinensis ginseng, towards commercializing products branded in Thai Nguyen province. Thereby promoting the production of medicinal herbs, sustainable agricultural products, restructuring crops, and fully exploiting the fund of upland land, single-crop rice land, and maize-growing land to take advantage of leisure labor resources, contributing to increasing the number of farmers’ income for people in the province. Creating commercial products associated with research and technology transfer.
Detail objective Developed a process to extract polysaccharides from Ginseng Dang Quy Angelica sinensis (Oliv.) Diels Testing and proving the activity of natural compounds obtained. Developed a formula to mix the obtained active ingredients and excipients to create instant tea products from Angelica sinensis ginseng root. Research questions and hypotheses Is there a way to disseminate Angelica Sinensis ginseng to consumers? Ginseng Angelica Sinensis is a precious medicine in oriental medicine with many good effects for the health of users. Now people can use Angelica Sinensis ginseng to cook nutritious dishes such as pork heart stew with Angelica Sinensis, and chicken stew with Angelica Sinensis.
The most used method is that of Angelica Sinensis soaked in alcohol. However, no studies have been conducted to produce tea from Angelica Sinensis ginseng. Dubbed the "Land of Tea", this is a potential opportunity for Thai Nguyen 4 province, both taking advantage of the effects of Angelica Sinensis and helping to create economic products for farmers. How to optimize the Beneficial Activities of Ginseng Angelica Sinensis? This study will conduct experiments to select the appropriate conditions for the extraction of Angelica sinensis and use the extract to create valuable products.4 Limitations Materials: Ginseng Angelica sinensis is a type of ginseng grown mainly in the northern mountainous provinces such as Lao Cai, Lai Chau, etc.
This leads to difficulties in purchasing and storing raw materials. During the transportation process, taking a long time can affect the input quality of ginseng. Besides, the relatively high cost of ginseng Angelica sinensis is also a hindrance to research. Physical facilities: The research facilities of the Faculty of Biotechnology and Food Technology at Thai Nguyen University of Agriculture and Forestry meet basic standards for conducting scientific research.
However, the design of the machine is different from other research facilities. The condition of the machine can cause errors in the conduct of the study. Besides, the origin of chemicals and other materials can completely affect the research results. Modern methods such as HPLC or Mass spectrometry are not yet within reach of students.
Natural conditions at the research site: The conditions in the research room of the Department of Biotechnology - Food Technology in particular and Thai Nguyen city in general at the time of the research were relatively hot, and the temperature was relatively hot average about 32-35 degrees.